mouse igf 1 (R&D Systems)
Structured Review
Mouse Igf 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+igf/Mouse+IGF-I%2FIGF-1+Antibody/10__3390_slash_ijms27083547-164-0-8
Average 93 stars, based on 117 article reviews
Images
Related Articles
Cell Culture:Article Title: Megakaryocytes co-localise with hemopoietic stem cells and release cytokines that up-regulate stem cell proliferation. Article Snippet: .. One hundred LSKSLAM cells and 100 MM were cultured in 100 μl of Iscove's Modified Dulbecco's media (IMDM, Life Technologies) supplemented with 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA) and recombinant human insulin (10 μg/ml, Novartis, Switzerland), human transferrin (100 μg/ml, Roche, USA), human low density lipoprotein (3 μg/ml, MP Biomedicals, Australia), L-glutamine (2 mM/ml, Life Technologies), human IL-11 (100 ng/ml, Chemicon, Australia), mouse stem cell factor (10 ng/ml, Chemicon), human IL-6 (10 ng/ml, Peprotech, USA), human FLT-3 ligand (10 ng/ml, Apollo Cytokine Research, Australia), mouse IL-3 (133 U/ml, Immunex Corporation, USA), human thrombopoietin (TPO) (5 ng/ml, Apollo Cytokine Research), with or without mouse IGFBP-3 (775-B3), mouse IGF-1 (791-MG) or Modification:Article Title: Megakaryocytes co-localise with hemopoietic stem cells and release cytokines that up-regulate stem cell proliferation. Article Snippet: .. One hundred LSKSLAM cells and 100 MM were cultured in 100 μl of Iscove's Modified Dulbecco's media (IMDM, Life Technologies) supplemented with 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA) and recombinant human insulin (10 μg/ml, Novartis, Switzerland), human transferrin (100 μg/ml, Roche, USA), human low density lipoprotein (3 μg/ml, MP Biomedicals, Australia), L-glutamine (2 mM/ml, Life Technologies), human IL-11 (100 ng/ml, Chemicon, Australia), mouse stem cell factor (10 ng/ml, Chemicon), human IL-6 (10 ng/ml, Peprotech, USA), human FLT-3 ligand (10 ng/ml, Apollo Cytokine Research, Australia), mouse IL-3 (133 U/ml, Immunex Corporation, USA), human thrombopoietin (TPO) (5 ng/ml, Apollo Cytokine Research), with or without mouse IGFBP-3 (775-B3), mouse IGF-1 (791-MG) or Article Title: Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM. Article Snippet: J. Mol.. Rec anical force detection for ligands bound to receptors on a cell surface, both of which are involved in a signal transduction pathway.. This pathway is an autocrine pathway, involving the production of insulin-like growth factor-II (IGF-II) and activation of the IGF-I receptor, involved in myoblast differentiation induced by MyoD in C3H10T1/2 mouse mesenchymal stem cells. Recombinant:Article Title: Megakaryocytes co-localise with hemopoietic stem cells and release cytokines that up-regulate stem cell proliferation. Article Snippet: .. One hundred LSKSLAM cells and 100 MM were cultured in 100 μl of Iscove's Modified Dulbecco's media (IMDM, Life Technologies) supplemented with 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA) and recombinant human insulin (10 μg/ml, Novartis, Switzerland), human transferrin (100 μg/ml, Roche, USA), human low density lipoprotein (3 μg/ml, MP Biomedicals, Australia), L-glutamine (2 mM/ml, Life Technologies), human IL-11 (100 ng/ml, Chemicon, Australia), mouse stem cell factor (10 ng/ml, Chemicon), human IL-6 (10 ng/ml, Peprotech, USA), human FLT-3 ligand (10 ng/ml, Apollo Cytokine Research, Australia), mouse IL-3 (133 U/ml, Immunex Corporation, USA), human thrombopoietin (TPO) (5 ng/ml, Apollo Cytokine Research), with or without mouse IGFBP-3 (775-B3), mouse IGF-1 (791-MG) or Article Title: Cysteine induces longitudinal bone growth in mice by upregulating IGF-I. Article Snippet: Cys, avidin-peroxidase and bovine serum albumin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). .. Article Title: Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM. Article Snippet: J. Mol.. Rec anical force detection for ligands bound to receptors on a cell surface, both of which are involved in a signal transduction pathway.. This pathway is an autocrine pathway, involving the production of insulin-like growth factor-II (IGF-II) and activation of the IGF-I receptor, involved in myoblast differentiation induced by MyoD in C3H10T1/2 mouse mesenchymal stem cells. |
![Effects of rexinoid treatment on macrophage gene and protein expression in the desiccation stress dry eye model and potential macrophage-derived <t>IGF-1/IGF-1R</t> signaling axis. ( A ) Heatmaps showing z -score–scaled expression of selected latent-time–associated genes in four treatment groups (NS, None [untreated], Veh, and NEt-3IB) in conjunctival monocyte/macrophage lineage cells sorted from CD45⁺ cells after 5 days of desiccating stress (DS5) ( P adj < 0.001). Genes are organized into five functional categories. The color scale represents z -scores of normalized expression ( blue , low; red , high). These data show desiccating stress–associated shifts in macrophage gene programs and their modulation by NEt-3IB, including enrichment of reparative and growth factor–related genes such as Igf1 . Full gene names for the abbreviations are provided in . ( B ) Representative flow cytometry histograms and quantification validating selected macrophage-associated markers in conjunctival immune cells. ( Left ) Representative histograms of CX3CR1 staining with fluorescence-minus-one (FMO) control, with corresponding quantification of the percentage of CD45⁺CD11b⁺CX3CR1⁺ cells. ( Right ) Representative histograms of IGF-1 staining with FMO control, with corresponding quantification of the percentage of CD45⁺CD11b⁺Mrc1⁺IGF-1⁺ cells. Compared with DS5 no treatment and DS5+vehicle controls, DS5+NEt-3IB increased the proportion ofx CX3CR1⁺ and Mrc1⁺IGF-1⁺ myeloid cells. Each dot represents one biological replicate; bars show mean ± SEM. Statistical significance is indicated as shown: ** P < 0.01; **** P < 0.001; **** P < 0.0001; ns, not significant. ( C ) Representative immunofluorescence images showing IGF-1R/WGA/DAPI staining of wholemount conjunctiva showing surface view ( top ) and βIII-tubulin/IGF-1R/DAPI staining in the cornea ( bottom ). IGF-1R localization is shown because IGF-1 is produced by ocular surface resident macrophages, suggesting a potential macrophage-derived IGF-1/IGF-1R signaling axis acting on ocular surface epithelial and neural compartments during DS. Scale bar : 100 µm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9652/pmc13089652/pmc13089652__iovs-67-4-31-f001.jpg)